Figure 6 | Scientific Reports

Figure 6

From: Hypoxia-induced reprogramming of glucose-dependent metabolic pathways maintains the stemness of human bone marrow-derived endothelial progenitor cells

Figure 6

ETC inhibition promoted cell proliferation under normoxia. (A) Proliferation assay of BM EPCs treated with vehicle or various concentration of AA under normoxia (n = 6). (B) Colony formation assay of BM EPCs treated with vehicle or 200 nM AA under normoxia (n = 6). (C) qRT-PCR showed higher expression of BM EPCs stemness markers in the presence of 200 nM AA than with vehicle control under normoxia (n = 5). (D) Representative experiment showing ECAR of BM EPCs and comparison of glycolytic rate and capacity in the presence or absence of AA under normoxia (n = 6). (E) Representative experiment showing OCR of BM EPCs and comparison of basal and maximal respiration in the presence or absence of AA under normoxia (n = 6). Data are presented as Mean ± SEM. *p < 0.05; **p < 0.01; *** p < 0.001 versus DMSO vehicle control.

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