Figure 1 | Scientific Reports

Figure 1

From: Overexpression of fatty acid synthase attenuates bleomycin induced lung fibrosis by restoring mitochondrial dysfunction in mice

Figure 1

FASN protein levels were decreased in the lungs of patients with IPF and in BLM-treated mice. (A) Immunohistochemical staining of FASN in human and mouse lungs. FASN was strongly localized in AECs (inset) in NC or control mice. Original magnification: × 100, inset panels × 400. (B) Lung FASN expression was measured by immunoblotting. Densitometry of FASN bands. Equal amounts of protein obtained from lung lysates were subjected to immunoblotting with anti–FASN Ab. Cropped images are displayed, uncropped blots are displayed in Supplementary Fig. 5. The data are expressed as the mean ± standard error of the mean. The data were normalized to β-actin. *P < 0.05, vs. NC or CTL. (C) Historical analysis of combination of immunofluorescence staining with anti-FASN and anti–SPC in IPF and NC (upper panels, n = 6 per group), CTL and BLM (lower panels, n = 6 per group). SPC-positive cells (green), FASN cells (red), and DAPI (blue) are shown. (D) Representative immunofluorescence images showing FASN (red) in AECIIs (using anti‐SPC, green) of NC, IPF lungs (upper panels, n = 6 per group), CTL and BLM lungs (lower panels, n = 6 per group). Scale bar, 50 µm. The data are expressed as the mean ± standard error of the mean. *P < 0.05, vs. NC or CTL.

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