Figure 7

FASN overexpression attenuates BLM- induced lung inflammation and fibrosis in a mouse model. (A) Protein expression of FASN was measured by immunoblotting. The intensity of FASN was quantified by densitometry, and the data were normalized to β-actin. Cropped images are displayed, uncropped blots are displayed in Supplementary Fig. 5. The data are expressed as the mean ± SEM. Error bars represent mean ± SEM (n = 6) *P < 0.05, vs. Doxy (−)/BLM (+). (B) H&E staining of control and BLM-treated FASN transgenic mouse lungs. Original magnification × 10; original magnification × 100 (C) Cell counts in BALF, collected on day 21. The total number of cells was counted using a hemocytometer. Differential cell counts in BALF were analyzed from 500 cells stained with Diff-Quick (n = 8 per group). (D) Masson’s trichrome staining in control and BLM-treated FASN transgenic mouse lungs. Original magnification × 200. (E) Quantification of lung fibrosis by Ashcroft score (n = 8 per group). Data are expressed as the mean ± SEM. (F) Collagen measurement by the hydroxyproline assay of control and BLM-treated mouse lungs (n = 8 per group). (G) Active TGF-β1 levels in control and BLM-treated mouse lungs (n = 8 per group). TGF-β1 levels in lung lysates were measured by ELISA. *P < 0.05 CTL/BLM(+) vs. FASN TG/BLM(+).