Figure 3
From: A set of vectors and strains for chromosomal integration in fission yeast
Vectors developed in this study. Structure of pCLys1 (a) and pCArg3 (b). The selection marker consists of the promoter and a part of the ORF of either lys1 (pCLys1) or arg3 (pCArg3). Both vectors have the nmt1 promoter and the ADH1 terminator for heterologous gene expression. Multicloning sites contain recognition sequences for NheI, XhoI, SmaI, BamHI, and AscI. An arrowhead indicates the NotI site for linearization of the plasmids. The schematic is not to scale.
