Figure 1
From: Proton and alpha radiation-induced mutational profiles in human cells
Overview of the experimental design. (a,b) Experimental identification of radiation dose in order to achieve ~ 50% of cell lethality (i.e., ~ 50% of cell survival) independently across the three cell lines and across the two types of radiations. SF, survival fraction. (c) Schematic overview of the experimental design of the study. A549, MCF7 and HAP-1 cell lines were exposed to GCR (protons or helium ions). GCR Galactic Cosmic Radiation. Single cells from treated and untreated conditions were separated by fluorescence-activated cell sorting (FACS) and clonally expanded. DNA from single-cell derived populations was extracted and subjected to whole genome sequencing.
