Figure 2

The expression of VDR, P66Shc, P-P66Shc and oxidative stress, fibrosis paramaters in AVF stenosis vessels. (A) IHC staining of VDR (left panels, magnification 20 × , right panel, magnification 400 ×) in the AVF stenosis patients and control subjects. (B) IHC staining of P66Shc (left panel), P-P66Shc (middle panel) and oxidative stress parameter, 8-ohdG (right panel) of two groups. (C) Western blot analysis of FN (upper panel), Col-1, P66Shc, P-P66Shc(middle panel) and VDR (bottom panel) protein expression in AVF vascular tissue of two groups . (D) Semiquantitative analysis of IHC stained with 8-ohdG, *P < 0.05 versus control groups, n = 3. (E–I) Densitometric semiquantitative analysis of the western blotting results, FN to β-actin (E), Col-1 to β-actin (F), P66Shc to β-actin (G), P-P66Shc to β-actin (H), VDR to β-actin (I), values were presented as the mean ± SD, *P < 0.05 versus control group, n = 3.