Figure 3

TGF-β induced FN, Col1, VDR, P66Shc expression and cellular morphologic changes in cultured HUVECs. (A) HUVECs morphology in different concentrations of TGF-β (magnification × 400). (B) Western blot analysis of FN (upper panel), Col-1, P66Shc, P-P66Shc (middle panel) and VDR (bottom panel) protein expression in HUVECs under different concentrations of TGF-β. C.CCK-8. assay to detect cell viability treated with different concentrations of TGF-β (10, 50 and 100 ng/ml) for 24-72 h. (D–H) Densitometric semiquantitative analysis of the western blotting results, FN to β-actin (D), Col-1 to β-actin (E), P66Shc to β-actin (F), P-P66Shc to β-actin (G), VDR to β-actin (H),*P < 0.05 versus control group, #P < 0.05 versus control group, n = 3. Data are presented as the mean ± SD.