Table 1 Box-Behnken for the optimization of process for IMOs production from saccharified starch using response surface methodology.

From: Immobilization of α-transglucosidase on silica-coated magnetic nanoparticles and its application for production of isomaltooligosaccharide from the potato peel

Run

Enzyme/substrate ratio (U g-1)

Time (Hours)

Temperature (°C)

pH

Isomaltose (g l-1)

Isomaltotriose (g l-1)

Panose (g l-1)

IMOs* (g l-1)

1

6.9

9

40

4.5

27.6

8.2

1.5

50

2

6.9

6

45

4.5

20

4.2

33.6

61

3

6.9

12

40

5.5

23

8

0

39

4

6.9

6

40

5.5

31.6

14.4

4.1

66

5

2.8

6

45

5.5

15

2.4

42

63

6

6.9

12

50

5.5

34

14

0

54

7

6.9

9

45

5.5

35.4

13.6

11.7

68

8

6.9

9

40

6.5

21

7

21

52

9

6.9

9

45

5.5

35.4

13.6

11.7

70

10

6.9

12

45

6.5

25

7.2

16.1

45

11

6.9

6

45

6.5

29.5

8.6

15.4

64

12

2.8

9

45

4.5

12.5

2.4

22.2

39

13

6.9

9

45

5.5

35.4

13.6

11.7

67

14

6.9

9

45

5.5

33.5

12.6

13.2

69.3

15

6.9

9

45

5.5

32

13

11

68

16

6.9

12

45

4.5

26

9

16.1

50.4

17

11

12

45

5.5

32.6

13.4

0

51.3

18

6.9

9

50

4.5

24

9

9

54

19

2.8

12

45

5.5

15

6

0

34

20

11

9

50

5.5

32.5

12

0

55

21

2.8

9

50

5.5

12.5

2.4

22.2

39

22

6.9

6

50

5.5

20.6

4.3

28

59.9

23

11

9

45

4.5

32.5

12

0

56

24

11

9

45

6.5

32.5

12

0

50.5

25

2.8

9

40

5.5

11.3

2.3

40

55

26

2.8

9

45

6.5

11.3

2.3

40

49

27

11

9

40

5.5

32.5

12

0

50.5

28

6.9

9

50

6.5

23.6

7.4

8

51

29

11

6

45

5.5

32.5

12.4

11

56

  1. *Total IMOs represents all isomaltooligosaccharide products including, kojibiose, nigerose, isopanose and DP > 3.
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