Figure 7
COX2 and ICAM1 are negatively regulated by miR-146a during HCEC inflammation. (A) The protein levels of COX2 and ICAM1 were measured by western blotting in the NC-m group, the TNF-α + NC-m group, the TNF-α + miR-146a-m group, the NC-i group, the TNF-α + NC-i group and the TNF-α + miR-146a-i group. The quantified data are shown in (B) and (C). (D) The secretion of sICAM1 protein in cell culture supernatant was measured by ELISA in the NC-m group, the TNF-α + NC-m group, the TNF-α + miR-146a-m group, the NC-i group, the TNF-α + NC-i group and the TNF-α + miR-146a-i group. The results are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. (E) The protein expression of ICAM1 was showed by the immunofluorescence staining in the NC-m group, the TNF-α + NC-m group, the TNF-α + miR-146a-m group and the TNF-α + NC-m + SC-514 group. Scale bars: 50 μm.
