Figure 9

Histologic analyses revealed no damage to the diaphragm. All panels represent a 10-times magnification. Panels (a–c) represent a cross-sectional view of the paced diaphragm. Panels (d–f) represent a longitudinal view of the paced diaphragm. Panels (g–i) represent the cross-sectional view of a healthy diaphragm and shows no difference compared to the paced diaphragm. In panels (a), (d), (g) Hematoxylin–eosin staining was used, in panels (b), (e), (h) Elastica-van Gieson staining was used, and in panels (c), (f), (i) Masson’s trichrome stain was used.