Figure 2

CSF bile acids associations with AD-implicated CSF protein clusters. Most pronounced associations collapsed into variable cluster components with the indicated Spearman’s rank order correlation statistics, including Spearman’s ρ and p-value. Two bile acids clusters were formed: one using GCDCA and TCDCA (upper two panels) and second using TDCA and GDCA (lower two panels). CSF proteins were organized into three clusters (Table S5): Cluster 1—containing mostly vascular proteins, with F2, AMBP and VTN as most representative members with R² > 0.8; Cluster 2—containing proteins involved in energy metabolism and cell proliferation, with PKM, ALDOA and PEBP1 as most representative members with R² > 0.7; Cluster 3 containing glycolysis regulating ENO2 and cell proliferation regulating COL6A1 and glycolytic enzyme ALDOC. Analysis was performed separately for AD cases and healthy controls and the two groups are indicated on the graph by colors: blue—healthy control; orange—AD cases. N for AD cases = 151; healthy controls = 142.