Figure 1 | Scientific Reports

Figure 1

From: Quantification of rare somatic single nucleotide variants by droplet digital PCR using SuperSelective primers

Figure 1

Diagram of the SP-ddPCR (SuperSelective primers in droplet digital PCR) optimization method to test SuperSelective primer accuracy and selective amplification of the allele of interest using DNA templates containing 10,000 molecules of the target loci bearing either the wild-type allele or the SNV. These measurements correspond to the estimation of 100% and 0% VAF by ddPCR. Suitable SuperSelective primers designed to detect APC c.4128T>A are shown as an example. WT, wild-type.

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