Figure 3 | Scientific Reports

Figure 3

From: Quantification of rare somatic single nucleotide variants by droplet digital PCR using SuperSelective primers

Figure 3

Validation of SNV-associated VAFs by SP-ddPCR in clinical patient samples. Plots show raw data output for fluorescence amplitude. Standard, total copies of the target gene measured by standard primers; SSP, quantification of SNV copies by SuperSelective primers; NTC, non-template control well containing equivalent amounts of DNA but not the target sequence. (a) Surrounding mucosa from APC c.4128T>A patient (b) Distal mucosa from APC c.4128T>A patient (c) CRC lesion from APC c.4128T>A patient (d) Surrounding mucosa from NRXN3 c.1421G>A patient (e) Distal mucosa from NRXN3 c.1421G>A patient (f) CRC lesion from NRXN3 c.1421G>A patient (g) Surrounding mucosa from NTRK2 c.220G>A patient (h) Distal mucosa from NTRK2 c.220G>A patient (i) AAD lesion from NTRK2 c.220G>A patient.

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