Table 3 Somatic variants analyzed with SuperSelective primers by ddPCR.
Chromosome | R† | A‡ | Gene Name | HGVS.c | WES total reads | WES variant reads | WES VAF | ddPCR VAF in SM§ | ddPCR VAF in DM¶ | ddPCR VAF in CL†† | ddPCR VAF in PBL‡‡ |
|---|---|---|---|---|---|---|---|---|---|---|---|
5 | T | A | APC | c.4128T>A | 63 | 4 | 6.35% | 0.09 ± 0.03% | 0.08 ± 0.05% | 5.61 ± 0.36% | 0.18 ± 0.07% |
5 | C | T | APC | c.2626C>T | 142 | 61 | 42.96% | 0.12 ± 0.05% | 0.15 ± 0.01% | 34.70 ± 0.73% | 0.08 ± 0.03% |
9 | G | A | LAMC3 | c.1241G>A | 152 | 11 | 7.24% | 2.51 ± 0.14% | 0.51 ± 0.09% | 0.14 ± 0.02% | 0.07 ± 0.05% |
14 | G | A | NRXN3 | c.1421G>A | 46 | 5 | 10.87% | 5.85 ± 0.45% | 4.02 ± 1.05% | 0.15 ± 0.05% | 0.13 ± 0.05% |
19 | C | T | ASNA1 | c.193C>T | 44 | 3 | 6.82% | 3.82 ± 0.19% | 0.12 ± 0.07% | 0.11 ± 0.03% | 0.32 ± 0.11% |
9 | G | A | NTRK2 | c.220G>A | 148 | 4 | 2.70% | 0.18 ± 0.03% | 0.21 ± 0.09% | 0.21 ± 0.09% | 0.39 ± 0.13% |
8 | C | T | FABP4 | c.105G>A | 146 | 4 | 2.74% | 0.02 ± 0.01% | 0.10 ± 0.06% | 0.06 ± 0.06% | 0.09 ± 0.03% |
