Figure 8
The knockdown of Nrf2 promoted oxaliplatin-induced ferroptosis in vitro. (A) The levels of GPX4 expression were increased in colon cancer tissues compared with normal tissues. (B) The survival curve showed a decreasing trend in the survival rate of colon cancer patients in the high GPX4 expression group. (C) The levels of GPX4 expression were increased in rectal cancer tissues compared with normal tissues. (D) The survival curve showed a decreasing trend in the survival rate of rectal cancer patients in the high GPX4 expression group. (E–H) By western blot, we found that oxaliplatin and lobaplatin inhibited the expression of GPX4. Compared with the control group, after knocking down Nrf2, oxaliplatin and lobaplatin reduced the expression of GPX4. (I, J) Oxaliplatin increased the MDA content. The knockdown of Nrf2 enhanced oxaliplatin to increase the MDA content. (K, L) Oxaliplatin decreased the GSH content. The knockdown of Nrf2 promoted oxaliplatin to reduce the GSH content. Data are shown as the mean ± SD, *p < 0.05; **p < 0.01; ***p < 0.001; ns (not significant).
