Figure 4 | Scientific Reports

Figure 4

From: Knockdown of Secernin 1 inhibit cell invasion and migration by activating the TGF-β/Smad3 pathway in oral squamous cell carcinomas

Figure 4

SCRN1 enhances MMP-9 exocytosis by activating the TGF-β/Smad3 pathway. (A) qPCR revealed that no significant difference in MMP-2/9 mRNA between control cells and SCNR1-knockdown cells. The data are presented as the mean ± SD, P > 0.05 and ns = no significant difference. (B) MMP-2 and MMP-9 protein secretion from SCRN1 knockdown SCC15 and HSC3 cells as determined by ELISA. The data are presented as the mean ± SD of three independent experiments. **p < 0.01. (C) Gelatinase activity as determined by zymogram densitometric analysis. The data are presented as the mean ± SD of three independent experiments. (D) After 48 h of treatment with TGF-β, MMP-2 and MMP-9 protein from SCRN1-knockdown SCC15 and HSC3 cells was detected by ELISA. The data are presented as the mean ± SD of three independent experiments. **p < 0.01.

Back to article page