Figure 3 | Scientific Reports

Figure 3

From: Quantitation and integrity evaluation of RNA genome in lentiviral vectors by direct reverse transcription-droplet digital PCR (direct RT-ddPCR)

Figure 3

Using 5 primers/probe sets to titrate lentivirus by detecting RNA genome copy number. (A) By using lentivirus genome DNA as template, 5 sets of primers/probe were all able to detect the viral genome DNA and showed equivalent PCR efficiency. (B–D) All 5 sets of primers/probe detected lentivirus RNA genome in one-step direct RT-ddPCR reactions. Sample C does not contain GFP gene, therefore there were only 4 assays displayed in Fig. 3D. Input copy number was determined by viral titer provided by the companies. All data presents mean ± SD, n = 3.

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