Table 1 Primer and probe information for ddPCR assays.
Primer/probe namea | DNA sequence | Amplicon size |
|---|---|---|
5′ LTR-F6 | TGCCCGTCTGTTGTGTGACT | 140 |
5′ LTR-R6 | CGAGTCCTGCGTCGAGAGA | |
5′ LTR-P | Fam-AGTCCCTGTTCGGGC-MGB | |
Psi-F6 | CTCTCTCGACGCAGGACTCG | 126 |
Psi-R6 | GACGCTCTCGCACCCATCT | |
Psi-P | VIC-CTCTTGCCGTGCGCG-MGB | |
RRE-F | AAACTCATTTGCACCACTGC | 106 |
RRE-R | AATTTCTCTGTCCCACTCCATC | |
RRE-P | Fam-TGTGCCTTGGAATGC-MGB | |
eGFP-F | TGCTGCCCGACAACCACT | 80 |
eGFP-R | CAGGACCATGTGATCGCG | |
eGFP-P | Fam-TGAGCAAAGACCCCAAC-MGB | |
WPRE-F | TTACGCTATGTGGATACGCTG | 109 |
WPRE-R | TCATAAAGAGACAGCAACCAGG | |
WPRE-P | VIC-AGGAGAAAATGAAAGCCATAC-MGB |
