Figure 4

Real-time PCR amplification curves specifically for influenza virus (A), co-infection H. influenzae (B), and B. subtilis + B. clausii (C) taken from representative nasopharyngeal sample of Control and Navax groups at day 0 and 2 of treatment; Reducing-fold levels (2△Ct) of influenza viral load (D) and concentration of co-infecting bacteria (E), and Threshold cycle (Ct) of fluorescent signals for B. subtilis and B. clausii (F) measured in nasopharyngeal samples of Control and Navax groups at day 2 compared to day 0. The Mann–Whitney test was used to calculate the median difference of these indicators in the two groups. 95% CI for median in each group and the median difference between the two groups were shown in Fig. 4D,F. The significance level of all analyzes was set at the p < 0.05.