Figure 5
From: Fibulin-3 is necessary to prevent cardiac rupture following myocardial infarction
Bulk RNA-seq reveals differential expression of ECM and muscle fibre organisation genes, inflammatory genes and Mmp9 in the infarct zone of Efemp1−/− at day-3 post-MI. (a) Bulk RNA-seq was conducted on isolated infarct zone tissue from WT mice (n = 7) and Efemp1−/− (n = 5) at day-3 post-MI. A condensed list of genes of interest are presented ranked by fold-change; edgeR analysis, all presented genes FDR ≥ 0.05. (b) Gene lists derived from RNA-seq underwent GSEA pathway analysis to identify pathways enriched in the WT and Efemp1−/− mice infarct zones. Pathway enrichment results were visualized in Cytoscape using the EnrichmentMap, AutoAnnotate and ClusterMaker2 apps before being manually laid out and labels removed for visual purposes. Enriched pathways for WT are shown in blue and the pathways enriched in Efemp1−/− mice are in red. Each node (circle) represents a distinct pathway, and edges (lines) represent overlapping genes between pathways, determined using a similarity coefficient. All the genes involved in the enriched pathways were then used to produce a heatmap, shown on the right.
