Figure 3
From: In vitro oxygen imaging of acellular and cell-loaded beta cell replacement devices
(A) The schematic of the experiment with fresh and refrigerated gelatin with 1 mM trityl OX071, the samples were deoxygenated by N2 bubbling and when completely deoxygenated in about an hour or so, the bubbling tube was pulled back, and 5% O2 was allowed to circulate on top of the sample, (B) the change in average pO2 for freshly prepared and refrigerated gelatin as a function of time, only reoxygenated part of the curve and images are shown here, (C) pO2 maps of fresh and refrigerated gelatin at t = 0, 4 h, 8 h, and 16 h, (D) A SONIC device embedded in 1% gelatin with 1 mM trityl OX071. The green color is because of the trityl OX071, (E) the change in average pO2 as a function of time for the control and the active device (n = 1), (F) pO2 maps at t = 0, 0.5 h, 1 h, and 8 h for control and active SONIC devices.
