Figure 2
From: EP300 promotes ferroptosis via HSPA5 acetylation in pancreatic cancer
EP300 positively regulates ferroptosis. (A) Western blot analysis of EP300 protein expression in EP300-knockdown PANC1 and SW1990 cells. (B) Measuring cell survival by cell count kit 8 following treatment with RSL3 (1 μM) for 24 h. (C) Cell death by PI/hoechst33342 staining in the indicated cells following treatment with RSL3 (1 μM) for 24 h. Scale bar: 100 μm. (D) Colony formation assay in indicated PDAC cells following treatment with RSL3 (1 μM) for 24 h; then an equal amount of surviving cells was re-cultured in 6-well plates for 14 days. (E) The lipid ROS level was assessed by flow cytometry using a C11-BODIPY probe in indicated cells following treatment with RSL3 (1 μM) for 24 h. (F) Measuring oxidative stress using an MDA assay kit in indicated cells following treatment with RSL3 (1 μM) for 24 h. (G) Cellular iron accumulation level was assayed by PGSK (Phen Green SK) probe, Fluorescence intensity was observed under the fluorescence microscope. Scale bar: 100 μm.
