Figure 3 | Scientific Reports

Figure 3

From: Design of a novel multiepitope vaccine against Chlamydia pneumoniae using the extracellular protein as a target

Figure 3

The final structure diagram of the vaccine. (A) CTLA-4 extracellular ___domain (red), linear B cell epitopes (blue), conformational B cell epitopes (bright yellow), HTL epitopes (green), and CTL epitopes (yellow). Prediction of secondary structure (B). Alpha helix, extended strand, beta-turn, and random coil accounted for 34.60%, 25.26%, 7.27%, and 32.87%, respectively. 3D vaccine models. (C) Original 3D model built by trRosetta server. (D) Final model refined using GalaxyRefine server. Gray represents random coil, cyan represents beta-sheet, green represents beta-turn, and red represents alpha-helix. Validation of 3D models (E–H). (E) The tertiary structure of the vaccine was verified by Prosa-Web, and the Z-score was − 7.93; (F) Plot of residue scores; (G) The blue part was the region of CTLA-4 extracellular ___domain, the red part was the region of EAAAK linker, and the yellow part was the region of the main sequence; (H) According to SWISS-MODEL, 96.52% of amino acid residues were in the favored regions of the Ramachandran plot.

Back to article page