Figure 6
From: Reconditioned monocytes are immunomodulatory and regulate inflammatory environment in sepsis
RM co-culture with PBMCs isolated from suspected sepsis patients. (A) Evaluation of oxidative stress in patient PBMCs in coculture with RM: Bar graphs showing the percent of cells producing ROS and the extent of ROS generation by cells in PBMCs cultures and PBMC-RM cocultures as quantified by DCFDA dye (N = 12). (B) Changes in cytokine secretory profile of cells in PBMCs cultures and PBMC-RM cocultures quantified by cytometric bead assay (N = 15). (C) Evaluation of apoptosis profile of patient PBMCs after coculture with RM: bar graphs showing the percent of viable cells and percent of cells in various phases of apoptosis in PBMCs cultures and PBMC-RM cocultures Quantified by Annexin-V/PI staining (N = 9). Results are presented as median with inter-quartile range.
