Figure 2
From: Generation of a familial hypercholesterolemia model in non-human primate
Deletion of LDLR expression in LDLR KO monkey. (a) Lysates of the liver isolated from control and LDLR KO monkeys were analyzed by western blotting. GAPDH was blotted as a loading control. (b) Immunohistochemical analysis of the liver and heart isolated from control and LDLR KO monkeys with the anti-LDLR antibody. The plasma membrane and nuclei were counterstained with wheat germ agglutinin (WGA) and DAPI, respectively. Scale bars: 100 μm. (c) Mutations in the LDLR gene of the generated monkeys. Various deletions and insertions in exon 2 and exon 4 were identified by the sequence analysis. In exon 4, a large nucleotide substitution that consisted of a 177 bp sequence including parts of intron 4 and exon 5 was also observed. At the beginning of this substitution, the stop codon (TGA) was occasionally present.
