Table 1 Type and frequency of mutations in the LDLR gene of monkey blastocysts by CRISPR/Cas9 genome editing.

From: Generation of a familial hypercholesterolemia model in non-human primate

 

Type of mutations caused by

gRNA #2 for exon 4

gRNA #3 for exon 4

gRNA #5 for exon 2

Blastocyst #1

No mutation (100%)

No mutation (14.3%)

2 bp deletion (100%)

5 bp deletion (85.7%)

Blastocyst #2

No mutation (100%)

2 bp insertion (20.0%)

16 bp insertion (66.7%)

6 bp deletion (80.0%)

5 bp deleion (33.3%)

Blastocyst #3

1 bp insertion (80%)

7 bp insertion (20.0%)

5 bp deletion (50.0%)

1 bp deletion (20%)

5 bp deletion (80.0%)

30 bp deletion (20.0%)

  

90 bp deletion (10.0%)

No mutation (20.0%)

Blastocyst #4

No mutation (100%)

3 bp deletion (100%)

4 bp deletion (100%)

Blastocyst #5

No mutation (100%)

3 bp deletion (100%)

20 bp insertion (100%)

Blastocyst #6

No mutation (100%)

6 bp deletion (100%)

1 bp nonsense mutation (100%)

  1. The percentage of mutations observed in the LDLR gene is indicated in ( ).
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