Figure 5
CAGE regulates tumorigenic potential in vivo. (a) The indicated cancer cells (each at 1 × 106) were injected into the dorsal flanks of athymic nude mice. Tumor volumes were measured as described. Significance determined by one-way ANOVA.***, p < 0.001. Each experimental group consisted of five athymic nude mice. (b) Tumor tissue lysates were subjected to immunoblot. Representative blots of three independent experiments were shown. The uncropped blots are shown in Supplementary Materials. (c) The indicated cancer cells (each at 1 × 106) were injected into the dorsal flanks of nude mice. Each experimental group consisted of five nude mice. Data are presented as a mean ± SEM. Tumor volumes were measured as described. Significance determined by one-way ANOVA. *, p < 0.05; ***, p < 0.001. (d) Tumor tissue lysates were subjected to immunoblot and immunohistochemical staining. Representative images of three independent experiments were shown. Data are presented as mean ± SEM. Significance determined by one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001. The uncropped blots are shown in Supplementary Materials.
