Figure 3 | Scientific Reports

Figure 3

From: Estimating residual undifferentiated cells in human chemically induced pluripotent stem cell derived islets using lncRNA as biomarkers

Figure 3

Detection the copy numbers using ddPCR and relatively expression using RT-PCR of selected markers. (a) The copy numbers of selected markers in 1 ng hCiPSCs RNA and 200 ng hCiPSC-islets RNA were measured by ddPCR. Each dot represented the fluorescence intensity of the droplet. The droplets can be divided into negative droplets and positive droplets according to their fluorescence amplitude. (b) The copy numbers of selected markers measured in the same input of hCiPSCs RNA. Results are presented as the mean ± standard deviation (n = 3). (c) RNA transcripts copy number of LNCPRESS2, LINC00678 and LOC105370482 in different samples, including human islets and five hCiPSC-islets. (d) Relative expression of LNCPRESS2, LINC00678 and LOC105370482 in four hCiPSCs lines, such as #C, #1003, #9 and #8, compared with hCiPSC-islets, respectively. Results are showed as the mean ± standard deviation (n = 3).

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