Figure 5 | Scientific Reports

Figure 5

From: Estimating residual undifferentiated cells in human chemically induced pluripotent stem cell derived islets using lncRNA as biomarkers

Figure 5

ddPCR detection results of selected marker in #8 cell spike-in samples. (a) Spike-in study to detection sensitivity of ddPCR-based method (for 0, 1, 3, 5, 10 and 20 hCiPSCs-#8 spiked in 106 hCiPSC-islet cells-#8), n = 3. (b) Pearson's correlation analysis showed RNA transcripts of hCiPSC-specific markers were positively correlated with hCiPSCs spike-in numbers in #8 cell lines. Results are presented as the mean ± standard deviation (n = 3). (c) Linear regression analysis and curve fitting for LNCPRESS2, LINC00678 and LOC105370482 in #8 cell mixtures, n = 3. (d) Calculation of sensitivity of hCiPSC-specific markers in #8 cell lines.

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