Figure 2
From: NARFL deficiency caused mitochondrial dysfunction in lung cancer cells by HIF-1α–DNMT1 axis
NARFL knockdown caused mitochondrial dysfunction via HIF-1α activation. (A) Knockdown NARFL increased HIF-1α expression and this effect was inhibited using siHIF-1α. (B–C) HIF-1α mRNA levels were also significantly increased in NARFL knockdown A549 (B) and H1299 (C) cells in normoxic/hypoxic conditions. (D–E) HIF-1α inhibition and 2ME2 treatment improved the effects of NARFL deficiency on ND genes in A549 (D) and H1299 (E) cells. F-I, HIF-1α inhibition and 2ME2 treatment also ameliorated the effects of NARFL deficiency on Complex I activity in A549 (F) and H1299 (G) cells, mtDNA copy number (H) and ATP levels (I). These results suggested that NARFL knockdown induced dysregulation of mitochondrial function via HIF-1α.
