Figure 4
From: Optimization of Plasmodium vivax infection of colonized Amazonian Anopheles darlingi

Scheme of mosquito treatment for DMFA with Plasmodium vivax-infected blood. Batches of approximately 100 An. darlingi were treated with a combination of para-aminobenzoic acid (PABA) supplement and/or antibiotics (PSG), daily after adult’s emergence and until day 14 post-feeding. Plasmodium vivax infected blood was offered via a DMFA to mosquito batches on the 4th day post emergence. Infected blood was divided in two aliquots. The first was offered to mosquitoes as whole blood (WB); and the second was prepared for serum replacement (SR) before being offered to the batches of the mosquitoes. Figure was generated using the following software: Power Point of Microsoft Office Package, Inkscape 1.3 (0e150ed6c4, 2023-07-21, dated July 2023) and Prism 10 for Windows 64-bit (Version10.0.2 Build 232).