Figure 5

Immuno-fluorescent localization of GnRH-I and ERα receptors in hypothalamic POA and PVN region at both 10X and 40X magnifications; (A & C) Diabetic condition reduced ir-GnRH-I and ir-ERα in both POA and PVN region of brain of mice, whereas C, WA and STZ + WA groups mice showing abundant expression of GnRH-I and ERα. Right upper corner shows expression of GnRH-I and ERα in POA at higher magnification. Arrows indicate the expression of both GnRH-I and ERα in POA and PVN regions. (B & D) Graph represents the mean values of integrated optical density (IOD) in arbitrary units (AU) in both POA and PVN regions of brain. Values are presented as mean ± SD, (n = 6), significance of difference is considered as p ≤ 0.05 (***p ≤ 0.001) with respect to indicated groups. (*** indicated the comparison between C and WA, STZ and STZ + WA; ^### indicates significant difference from the diabetic (STZ) group at p < 0.001. [C, Control; STZ, Streptozotocin; WA, Withaferin-A; STZ + WA, Streptozotocin + Withaferin-A].