Figure 6

Immuno-fluorescence localization of Caspase-3 and p53 in testicular cells of mice; (A & C) STZ-induced diabetic mice testis illustrates highest ir-Caspase-3 and ir-p53 in spermatogonial cells, whereas WA treatment testis shows weak immunoreactivity in all stages of testes in STZ + WA group at both 10X and 40X magnifications. Control and WA-treated mice testes have low expression for Caspase-3 and p53 in testicular cells. Arrows indicate the immunoreactivity of both Caspase-3 and p53 in testicular cells. (B & D) Graph represents the mean from immune-positive cells by integrated optical density (IOD) in arbitrary units (AU). Values are presented as mean ± SD, (n = 6), significance of difference is considered as p ≤ 0.05 (*p ≤ 0.05, ***p ≤ 0.001) with respect to indicated groups. (* and *** indicated the comparison between C and WA, STZ and STZ + WA; ^### indicate significant difference from the diabetic (STZ) group at p < 0.05, p < 0.01 and p < 0.001, respectively. [C, Control; STZ, Streptozotocin; WA, Withaferin-A; STZ + WA, Streptozotocin + Withaferin-A].