Figure 5
Assessment of α2A-AR stimulation on host defense. (A) Quantitative RT-PCR (qPCR) analysis of expression of antimicrobial peptides Lyz1, Defcr5 (vehicle control vs. UK 14,304 21 days P = 0.003), Reg3g (vehicle control vs. UK 14,304 21 days P = 0.027), Muc2 (vehicle control vs. UK 14,304 3 days P = 0.0002), and Tff3 (vehicle control vs. UK 14,304 3 days P < 0.0001) in ileum of mice treated with UK 14,304 or vehicle control via daily oral gavage for 3 or 21 days (n = 8). Data are shown as individual data points with median and interquartile range. (B) Representative pictures of alcian blue stainings of ileum samples of mice treated with vehicle or UK 14,304. (C) Representative pictures of immunofluorescent stainings for nuclei DAPI; blue) or Muc2 (green) of ileum samples of mice treated with vehicle or UK 14,304. (D) Quantification of (B). Data are shown as individual data points with median and interquartile range (n presents the mean number alcian blue positive cells of > 15 pictures of 8 individual mice). Vehicle control versus UK 14,304 21 days P < 0.0001. (E) Quantification of (C) showing fluorescent signal of Muc2 relative to DAPI. Data are shown as individual data points with median and interquartile range (n presents the mean number alcian blue positive cells of > 15 pictures of 8 individual mice). Vehicle control versus UK 14,304 21 days P < 0.0001. (F) Representative pictures of immunofluorescent stainings for nuclei (blue) and lysozyme (green). Statistical significance is indicated as follows: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
