Figure 1

(A) Schematic for acoustic tweezing cytometry (ATC) integrated with microscopic imaging, where the transducer was positioned as a 45° angle to avoid standing wave formation. (B) Integrin-anchored microbubbles functionalized with Arg-Gly-Asp (RGD) were subjected to acoustic radiation force associated with an ultrasound field. (C) Representative bright field image of a human embryonic stem cell (hPSC) colony with attached microbubbles (red arrowheads).