Figure 3

(A) Representative bright field images of microbubbles at specific time points actuated by ultrasound using different duty cycles (acoustic pressure 0.035 MPa). White circles represent the original positions of microbubbles before ultrasound pulse. (B) Microbubble displacement profiles recorded during the first five ultrasound pulses. (C) Schematics showing definition of peak and residual displacement respectively. (D–F) Average peak displacement (D), average residual displacement (E), and displacement integral (F). Box: 25–75%, bar-in-box: median, and whiskers: 1% and 99%. *p < 0.05, **p < 0.01, ***p < 0.001.