Figure 5

Construction of the ERSRGs Prognostic Signature in LUAD. (A, B) LASSO regression analysis visualized, pinpointing the most salient among the 56 ERSRG prognosis-associated genes, emphasizing the regularization and feature selection process. (C) A subsequent Multivariate Cox analysis on the 19 genes derived from LASSO, narrowing down to 11 genes with significant prognostic implications. (D) Bar chart representation of the 11 genes' Coef values, which formed the core of our prognostic signature. (E) Scatter plot elucidating the survival disparity between the high and low-risk groups within the training set, accentuating the risk-score's potency in stratifying patient outcomes. (F) Visual depiction of gene expression variations through a heat map, emphasizing the differential expression patterns of the 11 signature genes between the high and low-risk groups. This visualization was crafted with the assistance of the 'gplots' package (version 3.1.3) in R (version 4.2.1, [https://www.r-project.org/]). (G) Multivariate Cox analysis juxtaposing risk scores with clinical factors, corroborating the risk score's capacity as an independent prognostic determinant. (H) Kaplan–Meier overall survival curves, delineating the stark survival differential between LUAD patients stratified by their risk scores based on the prognostic signature. UA: Univariate Analysis, MA: Multivariate Analysis. (I) Time-dependent ROC curves, encapsulating the signature's predictive accuracy across 1-, 3-, and 5-year intervals within the TCGA cohort.