Figure 2

Verification of Cas12a activity for P. aeruginosadetection; (A) Fluorescence analysis of the feasibility of Cas12a for P. aeruginosadetection. (B) Real-time kinetic fluorescence analysis of target DNA induced Cas12a/crRNA cleavage. (C) Feasibility of RPA-assisted CRISPR/Cas12a lateral flow sensor for the detection of P. aeruginosa.