Figure 6
From: Moschus ameliorates glutamate-induced cellular damage by regulating autophagy and apoptosis pathway
Effects of Moschus on autophagy-related proteins and Glu-induced autophagy. (a) Effects of Moschus on Glu-induced Bclin1, p62, and LC3 protein expression by western blot analysis. 1: Control; 2: 20 mM Glu; 3: 3-MA + Glu; 4: Moschus-0.05 mg/ml + Glu; 5: Moschus-0.1 mg/ml + Glu. (b) Immunofluorescence staining of LC3 was performed. (c) The autolysosomes or autophagosomes were monitored by TEM assay. Black arrows: autophagosomes/autolysosomes, scale bars: 5 μm at × 3000, 2 μm at ×7000, and 500 nm at ×20,000. (d) The fluorescent dyes of MDC detected the autophagic vacuole. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the Glu-induced group.
