Table 4 Plasmids used in this study.

From: Metabolic engineering of Saccharomyces cerevisiae for second-generation ethanol production from xylo-oligosaccharides and acetate

Plasmids

Description

References

pRS42K

pRS42K, Kanamycin resistance gene

55

p426GPD

pRS426-PTDH3-TCYC1

Addgene (#14156)

Cas9-NAT

P414-PTEF1-Cas9-TCYC1-NAT1

Addgene (#64329)

p426-CDT-2

pRS425-PPGK1-CDT-2-TCYC1

56

gRNA-sor-K

pRS42K carrying SOR1 disruption gRNA cassette

This work

gRNA-gre-K

pRS42K carrying GRE3 disruption gRNA cassette

This work

p426-GH43-2/7

pRS426-PTDH3-GH43-7-TCYC1-PCCW12-GH43-2-TCYC1

This work

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