Figure 2

The analysis of organ indices and histological measurements in white and wild C. argus. (A), (D), & (G) The liver index, spleen index, and intestine index (organ weight/body weight × 100%) were examined for both white and wild C. argus, respectively (ANOVA, n = 10). (B), (E), & (H) The histology of liver (× 400), spleen (× 100), and intestine (× 40) was assessed through H&E staining in white and wild C. argus, respectively (n = 10). (C) The area of hepatic cells in the liver was measured and compared between the two varieties at 120 dpf and 150 dpf (ANOVA, n = 10). The average area of each liver cell in an individual is determined by dividing the area of liver tissue in the slice (excluding tissues like the pancreas and blood vessels) by the number of cell nuclei. (F) The ratio of red pulp to white pulp in the spleen was determined for both varieties at 120 dpf and 150 dpf (ANOVA, n = 10). The relevant ratio was obtained by dividing the area of the red pulp by the area of the white pulp. (I) The length of intestinal villus (IV) and muscular layer & serous membrane (MLSM) in the intestine was determined for both varieties at 120 dpf and 150 dpf (ANOVA, n = 10). All evaluations were performed using Image Processing and Analysis in Java (https://imagej.net/ij/), with the calculation rules for the corresponding area or length documented in the figure. Statistical significance was indicated by *, representing a significant difference (P < 0.05), or **, representing a highly significant difference (P < 0.01), between the two varieties.