Figure 6 | Scientific Reports

Figure 6

From: Moracin E and M isolated from Morus alba Linné induced the skeletal muscle cell proliferation via PI3K-Akt-mTOR signaling pathway

Figure 6

Mouse skeletal muscle cell differentiation and proliferation effect of moracin E and M. Evaluation of muscle cell differentiation by measuring myogenic protein expression. Evaluation of muscle cell proliferation by measuring phosphorylated proteins of PI3K/AKT/mTOR pathway. (A) 5 µM and 25 µM of moracin E were treated with differentiation culture media during the differentiation of C2C12. (B–H) Each protein level was determined using image J(software) following normalized by β-Actin level. (I) 5 µM and 25 µM of moracin M were treated during the differentiation of C2C12. (J–P) Each protein level was determined using image J(software) following normalized by β-Actin level. (n = 3; representative of 3 biological replicates per group). Original blots/gels are presented in Supplementary Figs. 12 and 13.

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