Figure 5
Source data is available upon request.
Immunisation-challenge study in calves with Nicotiana benthamiana Oo-ASP-1. Calves were immunised three times with either Nicotiana benthamiana Oo-ASP-1 + QuilA (n = 12) or QuilA alone (n = 12) prior to a trickle infection with L3-stage Ostertagia ostertagi. Evaluated parasitological parameters include (a) faecal egg output measured over time, (b) cumulative faecal egg output and (c) O. ostertagi worm counts at the time of necropsy. (d) The systemic IgG1 response to native Oo-ASP-1 in an ELISA was measured prior to immunisation (imm), one week after each immunisation and at necropsy, displayed in optical density 405 nm with background correction at 492 nm. (e) The mucosal IgG1 response to native Oo-ASP-1 was measured at time of necropsy, displayed in optical density 405 nm with background correction at 492 nm. Data are presented as mean values + / − standard error of the mean. P values for (b), (c) and (e) were calculated using a one-tailed Mann–Whitney test. P values for (d) were calculated using a Two-way ANOVA with Dunnet’s test for multiple comparison. Degrees of freedom for the numerator: 4. Degrees of freedom for the denominator: 110. F distribution value: 66.04. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 versus QuilA-adjuvant control. The experiments for all figures were conducted once.
