Figure 2

HH exposure induced ghrelin expression in the mouse stomach as well as plasma levels of ghrelin and acylated ghrelin. (A) Expression and distribution of ghrelin (red arrow) in the stomach of mice after HH exposure for 3 and 7 days; (B) Semiquantitative analysis of ghrelin expression in the stomach. (C,D) The contents of ghrelin and acylated ghrelin in the plasma of mice after HH treatment for 3 and 7 days. Values are displayed as the mean ± SEM (n = 6 mice), * P < 0.05, ** P < 0.01, *** P < 0.001 versus NN or indicated group.