Figure 5
Effects of coronary ligation and warming on aerobic and anaerobic enzymatic activities of cardiac ventricular and muscle tissues from coho salmon (Oncorhynchus kisutch). Enzymatic activities and thermal sensitivity of lactate dehydrogenase (LDH) from cardiac (A) and white muscle tissues (B), and the activities of Citrate synthase (CS) from cardiac tissues (C) and white muscle tissues (D). The data for coronary-ligated or sham-operated female (blue or red solid lines, respectively) and male (blue and red dashed lines, respectively) fish are depicted in each panel (see Table S5 for sample sizes across groups and temperatures). The inset tables depict the outcome from mixed models, which included the data obtained at 8, 15, 20, 25 and 30 °C. Only statistically significant (P ≤ 0.05) results are displayed here, see Table S6 for full disclosure of the statistical results. Asterisks (*) denote statistically significant differences between treatment groups (sham-operated vs. coronary-ligated). In (A), where a significant interaction between treatment group and experimental temperature (Treatment*Temperature) was detected, different capital and lower-case letters denote differences between the different temperatures within sham-operated or coronary-ligated groups, respectively. For variables where no significant Treatment*Temperature interaction was detected (A–D), different single lower-case letters denote differences between the different temperatures across treatment groups. Values are means ± SEM.
