Figure 6

Pathway enrichment analysis using KEGG Mapper mapped metabolite feature hits (number in parenthesis) with different biosynthesis and metabolic pathways (a) (minimuml compound hit values from 1 to 5 are not shown); Pathway impact analysis showing impact of significantly different pathways (p ≤ 0.05) influencing metabolic responses in plant leaves (b); Size of the circle indicates the impact of the pathway while the Color from red to yellow represents the high to low significance levels; Identified pathways were (1) steroid biosynthesis, (2) diterpenoid biosynthesis, (3) brassinosteroid biosynthesis, (4) sesquiterpenoid & triperpenoid biosynthesis, (5) one carbon pool by folate, (6) biosynthesis of secondary metabolites, (7) isoquinoline biosynthesis, (8) betainn biosynthesis, (9) linoleic acid metabolism, (10) vitamin 6 metabolism, (11) ubiquinone & other terpenoid biosynthesis, (12) pentose phosphate pathway, (13) carotenoid biosynthesis, (14) valine, leucine & isoleucine biosynthesis, (15) pentose & glucoronate interconversions, (16) tryptophan metabolism, (17). α-linoleic acid metabolism, (18) cutin, suberine & wax biosynthesis, (19) arginine & proline metabolism; Enrichment analysis of up-regulated 481 (c) and down regulated 548 (d) metabolite features obtained from FC threshold ≥ 2.0 into different compound groups using MetaboAnalyst 5.0.