Figure 4 | Scientific Reports

Figure 4

From: Enzyme mechanistic studies of NMA1982, a protein tyrosine phosphatase and potential virulence factor in Neisseria meningitidis

Figure 4

Biochemical characterization of NMA1982. (a) Dose–response inhibition of NMA1982 activity by the transition state-mimicking pan-PTP inhibitor sodium orthovanadate (Na3VO4). (b) NMA1982 activity, expressed as initial rate (V) in arbitrary fluorescence units (AFU) per minute, at various pH conditions as indicated. (c) NMA1982 activity at various temperatures as indicated. (d) Remaining NMA1982 activity in response to treatment with various concentrations of hydrogen peroxide for 1 or 2 h. (e) Remaining NMA1982 activity in response to treatment with 80 or 400 µM hydrogen peroxide for 1 h or 2 h with no DTT treatment or after rescue with 10 mM DTT. All data represent triplicate experiments and are presented as average ± SD.

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