Figure 5 | Scientific Reports

Figure 5

From: Enzyme mechanistic studies of NMA1982, a protein tyrosine phosphatase and potential virulence factor in Neisseria meningitidis

Figure 5

NMA1982 is recovered from the bacterial supernatant. Whole cell lysate and supernatant were analyzed by SDS-Page and immunoblotting. NMA1982 and PilE are present in the supernatant. PilE is used as a positive control since PilE is usually enriched in the bacterial supernatant. Rmp4 is an outer membrane protein thought to be enriched in outer membrane vesicles. Absence of Rmp4 in the supernatant suggests that the supernatants are not contaminated with membrane or vesicles. NADP glutamate dehydrogenase is a cytosolic enzyme used as a control. Interestingly, NADP glutamate dehydrogenase is recovered from a supernatant of an overnight culture of meningococci confirming that this protein is released during cell lysis (data not shown).

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