Table 1 Kinetic parameters of wild-type and mutant NMA1982 proteins.

From: Enzyme mechanistic studies of NMA1982, a protein tyrosine phosphatase and potential virulence factor in Neisseria meningitidis

 

Km (μM)

kcat (s−1)

kcat/Km (M−1 s−1)

NMA1982 wt

35

4.4 × 10−5

1.26

NMA1982 C95S

No activity detected

NMA1982 R100A

No activity detected

NMA1982 D71A

40

2.7 × 10−5

0.69

NMA1982 R136A

28

2.6 × 10−4

9.28

NMA1982 D71A/R136A

33

1.1 × 10−4

3.28

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