Figure 2
From: Inflammatory protein signatures in individuals with obesity and metabolic syndrome
Differential protein expression analysis between OBO and OBM. Differentially expressed proteins (DEPs) were identified from combined Olink inflammation and cardiometabolic panels (184 unique proteins), which was defined as DEPs with more than 1.25-fold change with a P-value < 0.05 and FDR < 0.05. (A) Hierarchical clustering based on all 184 proteins assayed using the two Olink panels showed a separation between obese unhealthy patients compared to obese with no metabolic syndrome. The heatmap shows z-scores with cut-off 4, and principal component analysis (PCA) confirmed the separation of the obese cases based on the expression profiles of all proteins (B). (C) A histogram displaying the number of DEPs across the two groups. The number of the DEPs relevant to all proteins assayed are stated in the panel. (D) A volcano plot summarizing DEPs based on log2 fold changes across the two groups. Red and blue circles show significantly differential-expressed proteins ≥ 0.6 log2 fold change (upregulated) or ≥  − 0.6 log2 fold change (downregulated), and p value ≥ 0.05. Differential expression analysis accounted for obesity as the main effect, while correcting for BMI, age, sex, and nationality. (E) OBM signature protein list.
